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http://adhlui.com.ui.edu.ng/jspui/handle/123456789/1887
Title: | Cloning and restriction mapping of the L-sorbose utilization genes from a clinical isolate of Escherichia coli (1) |
Authors: | OLUKOYA, D. K. |
Keywords: | Cloning Plasmid Escherichia coli L-sorbose |
Issue Date: | Sep-1993 |
Publisher: | Spectrum Books Limited |
Citation: | Afr. J. Med. med. Sci. (1993) 22(3): 13-15 |
Abstract: | About 30% of clinical isolates of Escherichia coli tested utilized L-sorbose as a carbon and energy source. Escherichia coli K-12 is naturally sorbose negative. The genes for L-sorbose utilization (sor*) is being used as a prototype for studying variable genes amongst bacterial pathogens. The sor* genes from seven isolates were transferable to E. coli K-12. The (sor*) region was cloned into plasmid pBR322 to give pDOK 1. Plasmid pDOK 1 is approximately 20kb in size. A restriction endonuclease map of pDOKl is presented. |
Description: | Article |
URI: | http://adhlui.com.ui.edu.ng/jspui/handle/123456789/1887 |
ISSN: | 1116-4077 |
Appears in Collections: | African Journal of Medicine and Medical Sciences |
Files in This Item:
File | Description | Size | Format | |
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Olukoya_Cloning_1993.pdf | Article | 5.32 MB | Adobe PDF | View/Open |
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