Please use this identifier to cite or link to this item: http://adhlui.com.ui.edu.ng/jspui/handle/123456789/1887
Title: Cloning and restriction mapping of the L-sorbose utilization genes from a clinical isolate of Escherichia coli (1)
Authors: OLUKOYA, D. K.
Keywords: Cloning
Plasmid
Escherichia coli
L-sorbose
Issue Date: Sep-1993
Publisher: Spectrum Books Limited
Citation: Afr. J. Med. med. Sci. (1993) 22(3): 13-15
Abstract: About 30% of clinical isolates of Escherichia coli tested utilized L-sorbose as a carbon and energy source. Escherichia coli K-12 is naturally sorbose negative. The genes for L-sorbose utilization (sor*) is being used as a prototype for studying variable genes amongst bacterial pathogens. The sor* genes from seven isolates were transferable to E. coli K-12. The (sor*) region was cloned into plasmid pBR322 to give pDOK 1. Plasmid pDOK 1 is approximately 20kb in size. A restriction endonuclease map of pDOKl is presented.
Description: Article
URI: http://adhlui.com.ui.edu.ng/jspui/handle/123456789/1887
ISSN: 1116-4077
Appears in Collections:African Journal of Medicine and Medical Sciences

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