P53 gene of chang-Iiver cells (Atee-Cell3) exposed to aflatoxin B1 (Afb): the effect of lysine on mutation at Codon 249 of Exon 7

Abstract

The effect of different regimes of lysine-pretreatment on mutation at the 3rd nucleotide base of codon 249 which is located at the 7th exon of p53 gene of Chang-liver cells (CC1L13) exposed to aflatoxin B1 (AFB1) has been investigated. There is an indication of inhibition of 1 ug/ml AFB1 - induced mutation by pretreatment of cells for 72 hr. with 5-moIar fold lysine equivalent of 1 ug/ml AFB| 1 u/g ml AFB1 was the does at which there was 50% survival among the cells of CC13 in cytotoxicity studies. The results suggest chemo prevention of AFB, induced mutation at codon 249 locus of Exon 7 in CCL13's p53 gene and by implication, maybe, AFB1-induced primary liver cancer.