Histological alteration and oxidative variables in Wistar rats with induced-sepsis: the protective effect of tomato pomace powder

Abstract

Background: Sepsis is a systemic inflammatory response to infection causing morbidity and mortality and has oxidative damage as one of the mechanisms. We induced sepsis in rats by caccal ligation and perforation (CLP) and then investigated the possible protective effect of tomato pomace powder (TPP) on the sepsis-induced neuropathy using vitamin E (VIT E) as a standard antioxidant. Methods: Thirty-nine male Wistar rats were randomized into six groups: Control (Cont) (N=5) received food and water; TPP (N=5) received TPP (50 mg/kg); V1T E (N=5) received VIT E (500 mg/ kg); CLP (N=8) had CLP; TPP+CLP (N=8) received TPP (50 mg/kg) plus CLP; VIT E+CLP (N=8) received VIT E (500 mg/kg) plus CLP. The CLP was done on first day while all other administration lasted 21 days after which, neurobehavioral tests were done, animals sacrificed and tissues processed for haematological, biochemical and histological tests. Results: The CLP group had significant (p< 0.05). Total white cell and neutrophil counts were significantly (p < 0.05) higher in the CLP group compared with the control group. Histological alterations induced by sepsis included degeneration of Purkinje, granule and pyramidal neurons which were ameliorated by TPP and VIT E. Conclusion: Our results indicated that TPP and VIT E demonstrated protective effects from brain damage caused by CLP-induced sepsis via maintenance of the anti-oxidant status.